This guide describes how to set up and operate the BD™ High Throughput Sampler (HTS) option with the BD™ LSR II, BD FACSCanto™, and BD FACSCanto™ II flow cytometers. You should know how to operate your flow cytometer before using the HTS option. For important safety information, refer to the safety booklet provided with the HTS option.

Throughput Sampler (HTS) option for the BD FACSCalibur™ multiple times with user-defined mixing, wash, and analysis Training. Interactive online tutorial.

… BD High Throughput sampler. The HTS supports standard 96 (u bottom plates best) and 384 well plates. It is operated using DiVA allowing users to define customized delivery protocols, mixing, wash and analysis parameters The BD LSRFortessa™ X-20 fits most flow cytometry analyzer needs, and can be ordered with up to 5 lasers-most commonly, blue, red, violet, yellow-green, and UV-which provides flexibility in laser wavelengths. This allows researchers to optimize assay design by selecting the latest fluorescent dyes and antibodies in experimental protocols. This is likely applicable to the Fortessa HTS as well; they’re very similar.

For important safety information, refer to the safety booklet provided with the HTS option. 6 BD LSRFortessa Cell Analyzer User’s Guide z BD High Throughput Sampler User’s Guide: Describes how to set up and operate the BD™ High Throughput Sampler (HTS) option. It also contains a description of BD FACSDiva software features specific to the HTS. z BD FACSFlow Supply System User’s Guide: … FORTESSA USER GUIDE COMPUTER SETUP: • Turn on computer before turning on the machine • Windows 10 Username: BDOperator • Password: BDIS#1 • Open Tera Term application on desktop and leave running in background • Log in to BD FACSDiva software MACHINE SETUP: • Turn … to)running)an)HTS)experiment.)The)HTS)uses)more)sheath)fluid)than)loading) thetubes.) 2. Switch)the)acquisition)control)switch)to)plate)mode.) 3. Remove)the)tube)of)DI)water)fromthe)SIT.) 4. Attach)the)HTS… Page 1 FACSFortessa – HTS User Instructions The BD™ High Throughput Sampler (HTS) option for the BD LSRFortessa™ cell analyzer provides rapid, fully automated sample acquisition from microtiter plates.

6 BD LSRFortessa Cell Analyzer User’s Guide z BD High Throughput Sampler User’s Guide: Describes how to set up and operate the BD™ High Throughput Sampler (HTS) option. It also contains a description of BD FACSDiva software features specific to the HTS. z BD FACSFlow Supply System User’s Guide: …

When the long clean is complete, run a fluidics shutdown and turn off the system. BD Fortessa HTS Procedure: 1) Remove the tube of water from the SIP and connect the HTS sample coupler. Leave the support arm to the side. 2) Choose “New Experiment” .

BD LSR Fortessa Flow Cytometry Analyzer equipped with HTS (High Throughput Sampler) BD FACSDiva HTS quick reference guide · HTS quick reference

When logged in, click the BD FACS DIVA software icon. 3. You will be prompted for a& 29 Aug 2014 Standard Operating Procedure For: Using the BD™ High Throughput Sampler ( HTS):. BD LSRII (L01/L02). Independent Training Manual FACSymphony A3 and BD FACSymphony A5 flow cytometers. The following list includes the available documentation for the system.

Log in to the computer (facsuser, password =BDIS) and open BD FACSDiva software. Log in to Diva at the prompt with your Diva username and password. 3. Turn on main power switch (Green button-right side) 4. BD ® High Throughput Sampler User’s Guide BD™ High Throughput Sampler (HTS) Customer Care Kit BD LSRFortessa™ X-30, BD LSRFortessa™ X-50, BD LSRFortessa™ X-30 Dual, BD FACSymphony™ A3, BD FACSymphony™ A5 and BD FACSymphony™ A3 Dual Flow Cytometers User’s Guide 1.
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The BD FACSCelesta multicolor cell analyzer helps rapidly and accurately analyze cells in a compact, affordable package.

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Genuttryck Omnibus; Kompletterande information; PDF-filer; Kompletterande the BD LSR II or BD LSR Fortessa and Aria I, respectively (BD Biosciences). difluoride (PVDF) membrane (Amersham Biosciences, Arlington Heights, IL).

When acquiring the entire 96-well plate is better to have only have one plate per experiment. … BD High Throughput sampler.

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† BD High Throughput Sampler User’s Guide: Describes how to set up and operate the BD® High Throughput Sampler (HTS) option. It also contains a description of BD FACSDiva software features specific to the HTS. † BD FACSFlow™ Supply System User’s Guide: Describes the optional automated sheath and waste fluid control system.

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BD LSR Fortessa X20 – How to Use Guide 4 3 At the top of the Browser panel, create a New Experiment (click the icon with a book). 4 Create a New Specimen (click the icon with a syringe). 5 Select Tube_001 (inside the syringe) by clicking in the green arrow. 6 Do 3 PRIMEs with the SIP 2 arm open and no tube installed. Then set the cytometer to

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Turn on the instrument: a. LSR Fortessa - red switch on the wall behind the monitors (Fig.